| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukocyte-associated immunoglobulin-like receptor 1; LAIR-1; hLAIR1; CD antigen CD305; LAIR1; CD305 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LAIR1 recombinant protein (Position: Q142-H287). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects leukocyte-associated immunoglobulin-like receptor 1 (gene LAIR1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 31.4 kDa, and the supplier reports an observed band at about 40 kDa.
LAIR-1 (CD305) is an inhibitory receptor of the immunoglobulin superfamily found on most leukocytes, including NK cells, T cells and B cells. It binds collagens and, after tyrosine phosphorylation, recruits the phosphatases SHP-1 and SHP-2 to dampen cell activation and cytotoxicity. It is studied as a regulator of immune homeostasis and as a potential immune checkpoint.
| Target | Leukocyte-associated immunoglobulin-like receptor 1 (gene LAIR1; UniProt Q6GTX8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 31.4 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human LAIR1 fragment (Gln142–His287), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places leukocyte-associated immunoglobulin-like receptor 1 in the cell membrane. Tissue expression noted by UniProt (human): Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
