| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Lamin B2/LMNB2 recombinant protein (Position: L128-E585). Human LMNB2 shares 81.8% amino acid (aa) sequence identity with mouse LMNB2. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects lamin-B2 (gene LMNB2) in human and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
Lamin B2 (LMNB2) is a B-type nuclear lamin, an intermediate filament protein that stays farnesylated and anchored to the inner nuclear membrane. Together with lamin B1 and A-type lamins it forms the nuclear lamina, which supports nuclear shape, chromatin organization and nuclear envelope reassembly after mitosis. Lamin B2 is important for neuronal migration in the developing brain.
| Target | Lamin-B2 (gene LMNB2; UniProt Q03252, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| ELISA | 0.1–0.5 µg/mL |
| Flow cytometry | Not specified by the supplier |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Lamin B2/LMNB2 fragment (Leu128–Glu585), expressed in E. coli. Sequence identity with the mouse ortholog: 81.8%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places lamin-B2 in the nucleus lamina.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
