| Field | Specification |
|---|---|
| Target | |
| Alternative names | Laminin subunit alpha-2;Laminin M chain;Laminin-12 subunit alpha;Laminin-2 subunit alpha;Laminin-4 subunit alpha;Merosin heavy chain;LAMA2;LAMM; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Laminin 2 alpha, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes laminin subunit alpha-2 (gene LAMA2) in rat, mouse and human samples and is validated for Western blot. The predicted molecular weight is 343.9 kDa, and the supplier reports an observed band at about 344 kDa.
Laminin alpha-2 (LAMA2, merosin) is the alpha chain of laminin-211 and laminin-221, the main laminins of the muscle and peripheral nerve basement membranes. It anchors muscle fibers to the extracellular matrix through dystroglycan and integrins, and LAMA2 mutations cause a form of congenital muscular dystrophy.
| Target | Laminin subunit alpha-2 (gene LAMA2; UniProt P24043, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 343.9 kDa |
| Observed MW | 344 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HeLa cells, A549 cells, PANC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human laminin 2 alpha. The peptide sequence is conserved in mouse. It differs from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. Tissue expression noted by UniProt (human): Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Roman Sydney et al. (2025) Collagen processing is essential for germ cell identity. Biology Open. 10.1242/bio.062198
