| Field | Specification |
|---|---|
| Target | |
| Alternative names | Laminin subunit beta-1;Laminin B1 chain;Laminin-1 subunit beta;Laminin-10 subunit beta;Laminin-12 subunit beta;Laminin-2 subunit beta;Laminin-6 subunit beta;Laminin-8 subunit beta;LAMB1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Laminin beta 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AODF-12 is a rabbit monoclonal antibody that recognizes laminin subunit beta-1 (gene LAMB1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 198.0 kDa, and the supplier reports an observed band at about 250 kDa.
Laminin beta-1 (LAMB1) is the beta chain found in many laminin isoforms, including laminin-111 and laminin-511, and is widely present in basement membranes. Laminins organize cells into tissues by binding cell surface receptors and other matrix proteins. In the developing brain LAMB1 helps maintain the basement membrane that anchors radial glia, and its loss disrupts cortical layering.
| Target | Laminin subunit beta-1 (gene LAMB1; UniProt P07942, human) |
|---|---|
| Clone | AODF-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 198.0 kDa |
| Observed MW | 250 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human laminin beta 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lu Naiyan et al. (2021) Black Phosphorus Nanoparticles Promote Osteogenic Differentiation of EMSCs Through Upregulated TG2 Expression. Nanoscale Research Letters. 10.1186/s11671-021-03610-2
Shi Wentao et al. (2020) Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro. Amino Acids. 10.1007/s00726-020-02918-0
Wentao Shi et al. (2019) Overexpression of TG2 enhances the differentiation of ectomesenchymal stem cells into neuron‑like cells and promotes functional recovery in adult rats following spinal cord injury. Molecular Medicine Reports. 10.3892/mmr.2019.10502
