Anti-Laminin gamma 1/LAMC1 Antibody Picoband®

SKU:BHA21006902
Research Validated
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
Click light‑blue chips for details
Anti-LAMC1 antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot and immunohistochemistry. Reactivity: Rat, Mouse, Human (species coverage varies by application). Recognizes laminin subunit gamma-1.
Target LAMC1
Host Rabbit
Reactivity Rat,Mouse,Human
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
Read full description · Open full gallery
Options selector
Catalog no. Size Conjugation
PA1581 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial — Unconjugated; 100 ug/vial — Carrier Free
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
      Form: Lyophilized
      Applications: IHC,WB
      Application details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
      Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial — Biotin; 100 ug/vial — Cy3; 100 ug/vial — Fluoro488; 100 ug/vial — Fluoro550; 100 ug/vial — Fluoro594; 100 ug/vial — FITC; 100 ug/vial — APC; 100 ug/vial — PE; 100 ug/vial — Fluoro647
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      Form: Liquid
      Applications: Flow Cytometry | WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cells
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial — HRP
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
      Form: Liquid
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target LAMC1
Alternative names Laminin subunit gamma-1;Laminin B2 chain;Laminin-1 subunit gamma;Laminin-10 subunit gamma;Laminin-11 subunit gamma;Laminin-2 subunit gamma;Laminin-3 subunit gamma;Laminin-4 subunit gamma;Laminin-6 subunit gamma;Laminin-7 subunit gamma;Laminin-8 subunit gamma;Laminin-9 subunit gamma;S-laminin subunit gamma;S-LAM gamma;LAMC1;LAMB2;
UniProt # P11047
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Rat
  • Mouse
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Laminin gamma 1, different from the related mouse sequence by one amino acid and rat sequence by three amino acids.
Molecular weight 177.6 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted, extracellular space, extracellular matrix, basement membrane.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) PA1581
Main SKU BHA21006902
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody detects laminin subunit gamma-1 (gene LAMC1) in rat, mouse and human samples and is validated for Western blot and IHC. The predicted molecular weight is 177.6 kDa, and the supplier reports an observed band at about 150–200 kDa.

Laminin gamma-1 (LAMC1) is the gamma chain present in most laminin isoforms, including laminin-111 and laminin-511, and so is found in nearly all basement membranes. Laminins bind integrins and dystroglycan to support cell adhesion, migration and tissue architecture. LAMC1 is essential for basement membrane assembly in the early embryo and is a common marker in extracellular matrix research.

Target Laminin subunit gamma-1 (gene LAMC1; UniProt P11047, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Rat, Mouse, Human
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers
Calculated MW 177.6 kDa
Observed MW 150–200 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 0.5–1 µg/mL (Human)
Western blot 0.1–0.5 µg/mL (Human, Rat, Mouse)

Samples with a confirmed band (WB): rat kidney tissue, rat lung tissue, U87 cells, SMMC cells, HeLa cells, SKOV1 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Samples with confirmed staining (IHC): human intestinal cancer tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Immunogen

Synthetic peptide from the C-terminal region of human laminin gamma 1. It differs from the mouse sequence at one residue.

Reactivity Notes

The supplier lists reactivity with rat, mouse and human. Tissue expression noted by UniProt (human): Found in the basement membranes (major component). The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide and thimerosal (a mercury compound) as preservatives. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Human, Rat, Mouse); IHC (paraffin sections) 0.5–1 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Rat, Mouse, Human. Other species are not listed by the supplier.
Q.Has this antibody been tested on canine samples?
A.Reactivity with canine samples has not been tested by the supplier. Aligning the immunogen sequence with the canine ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 177.6 kDa; observed bands at about 150 and 200 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide from the C-terminal region of human laminin gamma 1. It differs from the mouse sequence at one residue.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Zhongwang Yu et al. (2022) Microglia Regulate Blood–Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory Demyelination. Advanced Science. 10.1002/advs.202105442

Chunmeng Wang et al. (2024) The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation. International Journal of Molecular Sciences. 10.3390/ijms25021126

10% OFF
10% Off Pre-Designed siRNA Sets
SIRNA10
20% OFF
20% Off Transmembrane Proteins
TM20
$50 OFF
$50 Off All ELISA Kits
Limited time ELISA50
50% OFF
50% Off Lab Consumables + Free Shipping
LABSAVE50
Offer
Denarase® Endonuclease — 10% Off One Order
DENARASE10
FREE SAMPLE
Free Sample – CellTrypase Recombinant Trypsin-Like Enzyme
Limited time offer – availa... FREESAMPLE
25 kUFREE
Free 25 kU Sample — Denarase® & Denarase® High Salt
FREESAMPLE
FREE SAMPLE
Free Sample – MycoFold™ Growth Factors
Limited time offer – availa... Request Free Sample
15% OFF
15% Off Proteins from Trusted Suppliers — Limited Time
Limited Time PT15OFF
Browse all current deals
arrow_right [#fffff] Created with Sketch.
arrow_right [#fffff] Created with Sketch.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Get it NOW Free Sample
Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Get it NOW Free Sample
Try Celltrypse Free – Request Your Sample Today