| Field | Specification |
|---|---|
| Target | |
| Alternative names | Linker for activation of T-cells family member 1; 36 kDa phosphotyrosine adapter protein; pp36; p36-38; LAT |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LAT recombinant protein (Position: H28-E235). Human LAT shares 61% and 59.3% amino acid (aa) sequence identity with mouse and rat LAT, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes linker for activation of T-cells family member 1 (gene LAT) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 27.9 kDa, and the supplier reports an observed band at about 37 kDa.
LAT (linker for activation of T cells) is a transmembrane adaptor that becomes heavily tyrosine-phosphorylated after T-cell receptor engagement. Phosphorylated LAT recruits PLC-γ1, GRB2, GADS and other signaling proteins, linking the receptor to calcium mobilization, PKC and MAPK activation. It is essential for T-cell development and also functions in mast cell and NK cell receptor signaling.
| Target | Linker for activation of T-cells family member 1 (gene LAT; UniProt O43561, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 27.9 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human LAT fragment (His28–Glu235), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 61% and 59.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places linker for activation of T-cells family member 1 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
