| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lipopolysaccharide-binding protein; LBP; Lbp; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse LBP recombinant protein (Position: V26-R257). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects lipopolysaccharide-binding protein (gene Lbp) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 53.1 kDa, and the supplier reports an observed band at about 65 kDa.
Lipopolysaccharide-binding protein (LBP) is an acute-phase plasma protein made mainly by the liver. It binds the lipid A part of bacterial lipopolysaccharide and hands it to CD14, which then presents it to the TLR4/MD-2 receptor, greatly increasing cell sensitivity to Gram-negative bacteria. LBP is studied in innate immunity, sepsis and inflammation research.
| Target | Lipopolysaccharide-binding protein (gene Lbp; UniProt Q61805, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 53.1 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human A549 cells, human Raji cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse LBP fragment (Val26–Arg257), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes lipopolysaccharide-binding protein as secreted and also located in the cytoplasmic granule membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
