| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylcholine-sterol acyltransferase; 1-alkyl-2-acetylglycerophosphocholine esterase; Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase; Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; LCAT |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LCAT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25L42 is a rabbit monoclonal antibody that recognizes phosphatidylcholine-sterol acyltransferase (gene LCAT) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 49.6 kDa, and the supplier reports an observed band at about 50 kDa.
Lecithin–cholesterol acyltransferase (LCAT) is a plasma enzyme secreted mainly by the liver. On the surface of HDL and LDL particles it transfers a fatty acid from phosphatidylcholine to free cholesterol, forming cholesteryl esters that drive HDL maturation and reverse cholesterol transport. LCAT deficiency causes marked lipoprotein abnormalities, and the enzyme is studied in lipid metabolism and cardiovascular research.
| Target | Phosphatidylcholine-sterol acyltransferase (gene LCAT; UniProt P04180, human) |
|---|---|
| Clone | 25L42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.6 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): GES-1 cells.
Immunogen
Synthetic peptide based on the sequence of human LCAT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes phosphatidylcholine-sterol acyltransferase as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongliang Gao et al. (2026) Aberrant Cholesterol Metabolism Caused by Decreased Lecithin: Cholesterol Acyltransferase Promoted Hepatocarcinogenesis. Journal of Hepatocellular Carcinoma. 10.2147/JHC.S562838
