| Field | Specification |
|---|---|
| Target | |
| Alternative names | L-lactate dehydrogenase A chain;LDH-A;1.1.1.27;Cell proliferation-inducing gene 19 protein;LDH muscle subunit;LDH-M;Renal carcinoma antigen NY-REN-59;LDHA;PIG19; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LDHA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21L18 is a rabbit monoclonal antibody that recognizes L-lactate dehydrogenase A chain (gene LDHA) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 36.7 kDa, and the supplier reports an observed band at about 37 kDa.
Lactate dehydrogenase A (LDHA, LDH-M) is the muscle-type subunit of lactate dehydrogenase, a tetrameric enzyme that interconverts pyruvate and lactate while cycling NADH and NAD+. LDHA favors lactate production, supporting glycolysis when oxygen is limited and in rapidly dividing cells, so it is widely studied in cancer metabolism, the Warburg effect and hypoxia.
| Target | L-lactate dehydrogenase A chain (gene LDHA; UniProt P00338, human) |
|---|---|
| Clone | 21L18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.7 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HUH-7- WT cells, human HUH-7-LDHA KO cells, human HepG2 cells, human Jurkat cells, human HeLa cells, human PC-3 cells, rat heart, rat skin.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human LDHA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places L-lactate dehydrogenase A chain in the cytoplasm. Tissue expression noted by UniProt (human): Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhuxian Zhu et al. (2023) Microbiome and spatially resolved metabolomics analysis reveal the anticancer role of gut Akkermansia muciniphila by crosstalk with intratumoral microbiota and reprogramming tumoral metabolism in mice. Gut Microbes. 10.1080/19490976.2023.2166700
Jian Chen et al. (2020) The expression and role of glycolysis-associated molecules in infantile hemangioma. Life Sciences. 10.1016/j.lfs.2020.118215
