| Field | Specification |
|---|---|
| Target | |
| Alternative names | L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46; LDHB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LDHB |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23L43 is a rabbit monoclonal antibody that recognizes L-lactate dehydrogenase B chain (gene LDHB) in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 36.6 kDa, and the supplier reports an observed band at about 37kDa.
Lactate dehydrogenase B (LDHB, LDH-H) is the heart-type subunit of lactate dehydrogenase, which interconverts lactate and pyruvate using NADH and NAD+. LDHB-rich isoenzymes are abundant in heart and other oxidative tissues, where they favor converting lactate to pyruvate for mitochondrial use. LDHB is studied in energy metabolism and cancer metabolism research.
| Target | L-lactate dehydrogenase B chain (gene LDHB; UniProt P07195, human) |
|---|---|
| Clone | 23L43 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.6 kDa |
| Observed MW | 37kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:100 |
| Flow cytometry | 1:60 |
Immunogen
Synthetic peptide based on the sequence of human LDHB.
Reactivity Notes
The supplier lists reactivity with human. UniProt places L-lactate dehydrogenase B chain in the cytoplasm and mitochondrion inner membrane. Tissue expression noted by UniProt (human): Predominantly expressed in aerobic tissues such as cardiac muscle.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
