| Field | Specification |
|---|---|
| Target | |
| Alternative names | Left-right determination factor 2; Endometrial bleeding-associated factor; Left-right determination factor A; Protein lefty-2; Protein lefty-A; Transforming growth factor beta-4; TGF-beta-4; LEFTY2; EBAF; LEFTA; LEFTYA; TGFB4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LEFTY1 + LEFTY2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24L15 is a rabbit monoclonal antibody that recognizes left-right determination factor 2 (gene LEFTY2) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 40.9 kDa, and the supplier reports an observed band at about 34.44 kDa.
Lefty2 is a secreted TGF-β superfamily protein that antagonizes Nodal signaling. In the embryo it is expressed on the left side and restricts Nodal activity, which is needed for proper left–right asymmetry of internal organs. Lefty2 is also expressed in the endometrium, where it is studied in relation to the menstrual cycle.
| Target | Left-right determination factor 2 (gene LEFTY2; UniProt O00292, human) |
|---|---|
| Clone | 24L15 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.9 kDa |
| Observed MW | 34.44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Caco2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human LEFTY1 + LEFTY2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes left-right determination factor 2 as a secreted protein. Tissue expression noted by UniProt (human): Mesenchymal cells of the endometrial stroma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
