| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leptin; Obesity factor; Lep; Ob |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Leptin recombinant protein (Position: V22-C167). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects leptin in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 18.7 kDa, and the supplier reports an observed band at about 23 kDa.
Leptin (LEP) is a hormone secreted mainly by adipose tissue in proportion to fat mass. Acting through its receptor LEPR in the hypothalamus, it reduces food intake and increases energy expenditure, and it also influences reproduction, immunity and bone metabolism. Leptin deficiency causes severe obesity in mice and humans, and leptin is a key target in obesity and metabolic research.
| Target | Leptin (gene Lep; UniProt P41160, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 18.7 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): mouse small intestine tissue, mouse kidney tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat kidney tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse Leptin fragment (Val22–Cys167), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes leptin as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
D.G. Kishor Kumar et al. (2024) Involvement of ObRb receptor, nitric oxide, and BKCa channel signaling pathways in leptin-induced relaxation of pregnant mouse uterus. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176796
Yu-Si Cheng et al. (2010) Testis dysfunction by isoproterenol is mediated by upregulating endothelin receptor A, leptin and protein kinase Cɛ and is attenuated by an endothelin receptor antagonist CPU0213. Reproductive Toxicology. 10.1016/j.reprotox.2010.03.001
Chen Li et al. (2017) Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid. Endocrine. 10.1007/s12020-016-1212-2
Cheng Yu-Si et al. (2010) Stress-induced cardiac insufficiency relating to abnormal leptin and FKBP12.6 is ameliorated by CPU0213, an endothelin receptor antagonist, which is not affected by the CYP3A suppressing effect of erythromycin. Journal of Pharmacy and Pharmacology. 10.1211/jpp.61.05.0004
Zhou Ji-Yin et al. (2017) Trigonelline Inhibits Inflammation and Protects β Cells to Prevent Fetal Growth Restriction during Pregnancy in a Mouse Model of Diabetes. Pharmacology. 10.1159/000479088
