| Field | Specification |
|---|---|
| Target | |
| Alternative names | Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3; MAC2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Galectin-3/LGALS3 recombinant protein (Position: R129-I250). Human Galectin-3/LGALS3 shares 87.7% and 84.4% amino acid (aa) sequence identity with mouse and rat Galectin-3/LGALS3, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes galectin-3 (gene LGALS3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and ELISA.
Galectin-3 (LGALS3, Mac-2) is a chimeric galectin with a single carbohydrate-recognition domain and an N-terminal domain that allows it to form multimers. It is found in the cytoplasm, the nucleus and outside cells, where it affects cell adhesion, apoptosis, inflammation and fibrosis. Galectin-3 also marks damaged endolysosomes, and it is widely studied in heart failure, fibrosis, cancer and macrophage biology.
| Target | Galectin-3 (gene LGALS3; UniProt P17931, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human Galectin-3/LGALS3 fragment (Arg129–Ile250), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 87.7% and 84.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes galectin-3 as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): A major expression is found in the colonic epithelium.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
