| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukemia inhibitory factor; LIF; Differentiation-stimulating factor; D factor; Lif |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse LIF recombinant protein (Position: S24-F203). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes leukemia inhibitory factor (gene Lif) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 22.3 kDa, and the supplier reports an observed band at about 18 kDa.
Leukemia inhibitory factor (LIF) is an IL-6 family cytokine that signals through a receptor made of LIFR and gp130, activating JAK–STAT3 signaling. It maintains self-renewal of mouse embryonic stem cells in culture and is essential for embryo implantation. LIF also acts on neurons, bone, muscle and immune cells and is widely used in stem cell culture and cytokine research.
| Target | Leukemia inhibitory factor (gene Lif; UniProt P09056, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 22.3 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, rat heart tissue, rat liver tissue, rat kidney tissue, human HeLa cells, human placenta tissue, human Jurkat cells, mouse liver tissuemouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse LIF fragment (Ser24–Phe203), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes leukemia inhibitory factor as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiyuan Dong et al. (2016) MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes formation during embryo implantation in mice. American Journal of Translational Research. PMID: 27158401
Dongmei Lai et al. (2010) Optimization of Culture Conditions to Support Undifferentiated Growth of Human Embryonic Stem Cells. Cellular Reprogramming. 10.1089/cell.2009.0106
