| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; ILT-2; Monocyte/macrophage immunoglobulin-like receptor 7; MIR-7; CD antigen CD85j; LILRB1; ILT2; LIR1; MIR7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LILRB1 recombinant protein (Position: Q347-Q442). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets leukocyte immunoglobulin-like receptor subfamily B member 1 (gene LILRB1) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 70.8 kDa, and the supplier reports an observed band at about 71 kDa.
LILRB1 (ILT2, LIR-1, CD85j) is an inhibitory immune receptor found on NK cells, T cells, B cells and monocytes. It binds a broad range of HLA class I molecules, including HLA-G, and the cytomegalovirus decoy UL18, and delivers inhibitory signals through ITIM motifs. LILRB1 is studied in NK cell regulation, maternal–fetal tolerance and as an immune checkpoint in cancer.
| Target | Leukocyte immunoglobulin-like receptor subfamily B member 1 (gene LILRB1; UniProt Q8NHL6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 70.8 kDa |
| Observed MW | 71 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human lymphoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human LILRB1 fragment (Gln347–Gln442), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places leukocyte immunoglobulin-like receptor subfamily B member 1 in the cell membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in B cells, monocytes and various dendritic cell (DC) subsets including myeloid, plasmacytoid and tolerogenic DCs (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Feng Wang et al. (2010) Expression change of PirB in mice retina after optic nerve injury. Molecular Medicine Reports. 10.3892/mmr_00000272
