| Field | Specification |
|---|---|
| Target | |
| Alternative names | LIM domain kinase 1; LIMK-1; LIMK1; LIMK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LIM Kinase 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20L63 is a rabbit monoclonal antibody that recognizes LIM domain kinase 1 (gene LIMK1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 72.6 kDa, and the supplier reports an observed band at about 73 kDa.
LIM kinase 1 (LIMK1) is a serine/threonine kinase that controls actin dynamics downstream of Rho family GTPases. After activation by ROCK or PAK, it phosphorylates and inactivates cofilin, which stabilizes actin filaments. LIMK1 is studied in cell migration, dendritic spine and synapse biology, and it is one of the genes deleted in Williams syndrome.
| Target | LIM domain kinase 1 (gene LIMK1; UniProt P53667, human) |
|---|---|
| Clone | 20L63 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 72.6 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human LIM Kinase 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places LIM domain kinase 1 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Highest expression in both adult and fetal nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
