| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein lin-28 homolog B;Lin-28B;LIN28B;CSDD2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Lin28B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EAA-12 is a rabbit monoclonal antibody that recognizes protein lin-28 homolog B (gene LIN28B) in human samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 27.1 kDa, and the supplier reports an observed band at about 35 kDa.
LIN28B is an RNA-binding protein and paralog of LIN28A that suppresses production of let-7 microRNAs. It binds primary let-7 transcripts in the nucleus and keeps them from being processed, which supports stem cell self-renewal and fetal growth programs. LIN28B is studied in development, fetal hematopoiesis, puberty timing and cancers such as neuroblastoma.
| Target | Protein lin-28 homolog B (gene LIN28B; UniProt Q6ZN17, human) |
|---|---|
| Clone | EAA-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.1 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human K562 cells, human 293T cells, human HepG2 cells, human HUH-7 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Immunogen
Synthetic peptide based on the sequence of human lin28B.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein lin-28 homolog B in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Cheng-cheng Wang et al. (2016) Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1β/miR-101/Lin28B Signaling Pathway. Integrative Cancer Therapies. 10.1177/1534735416659358
