| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet-activating factor acetylhydrolase IB subunit beta; Lissencephaly-1 protein; LIS-1; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; PAFAH alpha; PAFAH1B1; LIS1; MDCR; MDS; PAFAHA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LIS1/PAFAH1B1 recombinant protein (Position: I95-R410). Human PAFAH1B1 shares 100% amino acid (aa) sequence identity with both mouse and rat PAFAH1B1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects platelet-activating factor acetylhydrolase IB subunit beta (gene PAFAH1B1) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 46.6 kDa, and the supplier reports an observed band at about 47 kDa.
LIS1 (PAFAH1B1) is a WD-repeat protein that regulates the microtubule motor dynein and is also the regulatory subunit of the enzyme PAF acetylhydrolase IB. It is required for nuclear movement and neuronal migration in the developing cortex, and mutations in the gene cause lissencephaly.
| Target | Platelet-activating factor acetylhydrolase IB subunit beta (gene PAFAH1B1; UniProt P43034, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.6 kDa |
| Observed MW | 47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human LIS1/PAFAH1B1 fragment (Ile95–Arg410), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places platelet-activating factor acetylhydrolase IB subunit beta in the cytoplasm and nuclear membrane. Tissue expression noted by UniProt (human): Fairly ubiquitous expression in both the frontal and occipital areas of the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
