| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor ligand superfamily member 4; OX40 ligand; OX40L; CD252; Tnfsf4; Ox40l; Txgp1l |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Livin/Birc7 recombinant protein (Position: Q41-S285). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Livin/Birc7 Antibody Picoband® is an antibody for Birc7 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Birc7 (tumor necrosis factor (ligand) superfamily, member 4); UniProt: A2AWP0
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 33 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Livin/Birc7 Antibody Picoband® catalog # A02577-2.
Biological background
Biological context: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Expression and localization notes: cellular localization: Membrane; Single-pass type II membrane protein., tissue context: Macrophages; peripheral blood leukocytes, lung, spleen and liver..
Common research applications
- Western blotting (WB): Compare Birc7 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Birc7 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Birc7-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Baculoviral IAP repeat-containing protein 7 is a protein that in humans is encoded by the BIRC7 gene. This protein is a member of the family of inhibitor of apoptosis proteins(IAP) and contains a single copy of a baculovirus IAP repeat(BIR) and a RING finger motif. Its gene is mapped to 20q13.3. It has got 280- and 298- amino acids. The protein is highly expressed in melanomas while lowly expressed in some lymphomas, fetal kidney, fetal liver, testis and thymus. BIRC7 plays a vital role in blocking apoptosis induced.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane; Single-pass type II membrane protein.
- Tissue details: Macrophages; peripheral blood leukocytes, lung, spleen and liver.
- Research category: Adaptive Immunity,Autoimmune,B Cells,Cell Type Markers,Cytokines,Immune System Diseases,Immunology,Innate Immunity,T Cells,TNF Superfamily
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
