| Field | Specification |
|---|---|
| Target | |
| Alternative names | Baculoviral IAP repeat-containing protein 7; Livin; RING-type E3 ubiquitin transferase BIRC7; Birc7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Livin/Birc7 recombinant protein (Position: Q41-S285). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes baculoviral IAP repeat-containing protein 7 (gene Birc7) in mouse samples and is validated for Western blot and ELISA. The predicted molecular weight is 32.0 kDa, and the supplier reports an observed band at about 33 kDa.
Livin (BIRC7, also called ML-IAP) is a member of the inhibitor of apoptosis family with a single BIR domain and a RING domain. It can inhibit caspases and bind SMAC, and it is expressed in melanoma and several other cancers, where it is studied in resistance to apoptosis.
| Target | Baculoviral IAP repeat-containing protein 7 (gene Birc7; UniProt A2AWP0, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 32.0 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse heart tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Livin/Birc7 fragment (Gln41–Ser285), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places baculoviral IAP repeat-containing protein 7 in the nucleus, cytoplasm and Golgi apparatus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
