| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase STK11;2.7.11.1;Liver kinase B1;LKB1;hLKB1;Renal carcinoma antigen NY-REN-19;STK11;LKB1, PJS; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LKB1 recombinant protein (Position: K62-C430). Human LKB1 shares 89% amino acid (aa) sequence identity with both mouse and rat LKB1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets serine/threonine-protein kinase STK11 in human samples and is validated for Western blot. The predicted molecular weight is 48.6 kDa, and the supplier reports an observed band at about 55 kDa.
LKB1 (STK11) is a tumor suppressor serine/threonine kinase that activates AMPK and a family of AMPK-related kinases by phosphorylating their activation loop. Working with STRAD and MO25, it links cellular energy status to metabolism, growth control and cell polarity. Germline STK11 mutations cause Peutz-Jeghers syndrome, and somatic loss is common in lung cancer.
| Target | Serine/threonine-protein kinase STK11 (gene STK11; UniProt Q15831, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 48.6 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human HeLa cells, human HepG2 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human LKB1 fragment (Lys62–Cys430), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 89%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein kinase STK11 in the nucleus, cytoplasm, membrane and mitochondrion. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yi Tang et al. (2015) ART1 promotes starvation-induced autophagy: a possible protective role in the development of colon carcinoma. American Journal of Cancer Research. PMID: 25973293
Yezi Kong et al. (2022) FGF21 Reduces Lipid Accumulation in Bovine Hepatocytes by Enhancing Lipid Oxidation and Reducing Lipogenesis via AMPK Signaling. Animals. 10.3390/ani12070939
Lv Yue et al. (2024) Dapagliflozin promotes browning of white adipose tissue through the FGFR1-LKB1-AMPK signaling pathway. Molecular Biology Reports. 10.1007/s11033-024-09540-3
Kuiyuan Song et al. (2018) Liver kinase B1 suppresses growth of lung cancer cells through sonic hedgehog signaling pathway. Cell Biology International. 10.1002/cbin.10965
