| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucyl-cystinyl aminopeptidase; Cystinyl aminopeptidase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP; Oxytocinase; OTase; Placental leucine aminopeptidase; P-LAP; LNPEP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LNPEP recombinant protein (Position: E17-T994). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets leucyl-cystinyl aminopeptidase (gene LNPEP) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 117.3 kDa, and the supplier reports an observed band at about 117 kDa.
LNPEP (insulin-regulated aminopeptidase, IRAP, also known as oxytocinase) is a zinc aminopeptidase that removes N-terminal amino acids from peptide hormones, including oxytocin, angiotensin III and vasopressin. It is present in GLUT4-containing vesicles of fat and muscle cells and moves to the cell surface in response to insulin. LNPEP is studied in peptide hormone metabolism and antigen cross-presentation.
| Target | Leucyl-cystinyl aminopeptidase (gene LNPEP; UniProt Q9UIQ6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 117.3 kDa |
| Observed MW | 117 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human Daudi cells, human T-47D cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human LNPEP fragment (Glu17–Thr994), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leucyl-cystinyl aminopeptidase in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in placenta, heart, kidney and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qian Ma et al. (2023) Leucyl and Cystinyl Aminopeptidase as a Prognostic-Related Biomarker in OV Correlating with Immune Infiltrates. Pharmacogenomics & Personalized Medicine. 10.2147/PGPM.S400145
