| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oxidized low-density lipoprotein receptor 1;Ox-LDL receptor 1;C-type lectin domain family 8 member A;Lectin-like oxidized LDL receptor 1;LOX-1;Lectin-like oxLDL receptor 1;hLOX-1;Lectin-type oxidized LDL receptor 1;Oxidized low-density lipoprotein receptor 1, soluble form;OLR1;CLEC8A, LOX1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human LOX-1/OLR1, different from the related rat sequence by thirteen amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes oxidized low-density lipoprotein receptor 1 (gene OLR1) in human samples and is validated for Western blot. The predicted molecular weight is 31.0 kDa, and the supplier reports an observed band at about 38 kDa.
LOX-1 (OLR1) is a C-type lectin scavenger receptor on endothelial cells, macrophages and smooth muscle cells that binds and internalizes oxidized LDL. Its activation triggers reactive oxygen species production, NF-κB signaling and endothelial dysfunction, so LOX-1 and its soluble form are widely studied in atherosclerosis and vascular inflammation.
| Target | Oxidized low-density lipoprotein receptor 1 (gene OLR1; UniProt P78380, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 31.0 kDa |
| Observed MW | 38 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human LOX-1/OLR1. It differs from the rat sequence at thirteen residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes oxidized low-density lipoprotein receptor 1 as secreted and also located in the cell membrane and membrane raft. Tissue expression noted by UniProt (human): Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
