| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; Olr1; Lox1; Oldlr1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat LOX 1/Olr1 recombinant protein (Position: Q55-Q364). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes oxidized low-density lipoprotein receptor 1 (gene Olr1) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 41.9 kDa, and the supplier reports an observed band at about 52 kDa.
LOX-1 (OLR1) is a C-type lectin scavenger receptor on endothelial cells, macrophages and smooth muscle cells that binds and internalizes oxidized LDL. Its activation triggers reactive oxygen species production, NF-κB signaling and endothelial dysfunction, so LOX-1 and its soluble form are widely studied in atherosclerosis and vascular inflammation.
| Target | Oxidized low-density lipoprotein receptor 1 (gene Olr1; UniProt O70156, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 41.9 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse brain tissue, mouse liver tissue, mouse kidney tissue, mouse smooth muscle tissue, mouse SP2/0 cells, mouse Neuro-2a cells, rat brain tissue, rat liver tissue, rat kidney tissue, rat smooth muscle tissue, rat C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Neuro-2a cells.
Immunogen
Recombinant rat LOX 1/Olr1 fragment (Gln55–Gln364), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes oxidized low-density lipoprotein receptor 1 as secreted and also located in the cell membrane and membrane raft. Tissue expression noted by UniProt (rat): Predominantly expressed in lung and at lower level in kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yin Lin et al. (2014) Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats. Food and Chemical Toxicology. 10.1016/j.fct.2014.03.026
