| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein-lysine 6-oxidase;1.4.3.13;Lysyl oxidase;LOX; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LOX |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABEO-12 is a rabbit monoclonal antibody that recognizes protein-lysine 6-oxidase (gene LOX) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 46.9 kDa, and the supplier reports an observed band at about 47 kDa.
Lysyl oxidase (LOX) is a copper-dependent secreted enzyme that oxidizes lysine and hydroxylysine residues in collagen and elastin. The resulting aldehydes form covalent cross-links that give connective tissues tensile strength and elasticity, and LOX is needed for the structural integrity of the aorta. LOX is studied in fibrosis, vascular biology and cancer metastasis.
| Target | Protein-lysine 6-oxidase (gene LOX; UniProt P28300, human) |
|---|---|
| Clone | ABEO-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.9 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human SiHa cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human lung squamous cells carcinoma tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Jurkat cells.
Immunogen
Synthetic peptide based on the sequence of human LOX.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes protein-lysine 6-oxidase as a secreted protein. Tissue expression noted by UniProt (human): Heart, placenta, skeletal muscle, kidney, lung and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuanbo Jia et al. (2021) The Plasticity of Nanofibrous Matrix Regulates Fibroblast Activation in Fibrosis. Advanced Healthcare Materials. 10.1002/adhm.202001856
Jie Yi et al. (2022) Insights into the interaction of cyclooxygenase and lipoxygenase with natural compound 3,4′,5,7-Tetrahydroxyflavone based on multi-spectroscopic and metabolomics. Spectrochimica ACTA Part a-Molecular and Biomolecular Spectroscopy. 10.1016/j.saa.2022.121800
Lijun Ou et al. (2026) Buyang Huanwu Decoction Alleviates Chronic Intermittent Hypoxia–Induced Myocardial Inflammation and Fibrosis via the NF-κB/LOX Signaling Pathway. Human Mutation. 10.1155/humu/2462859
