| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysyl oxidase homolog 2;1.4.3.13;Lysyl oxidase-like protein 2;Lysyl oxidase-related protein 2;Lysyl oxidase-related protein WS9-14;LOXL2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human LOXL2, different from the related mouse and rat sequences by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets lysyl oxidase homolog 2 (gene LOXL2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 86.7 kDa, and the supplier reports an observed band at about 105 kDa.
Lysyl oxidase-like 2 (LOXL2) is a copper-dependent amine oxidase that cross-links collagen and elastin in the extracellular matrix. Inside the nucleus it can also oxidize trimethylated histone H3 lysine 4 and act as a transcriptional corepressor, including in epithelial–mesenchymal transition. LOXL2 is studied in fibrosis, tissue stiffness and tumor progression.
| Target | Lysyl oxidase homolog 2 (gene LOXL2; UniProt Q9Y4K0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 86.7 kDa |
| Observed MW | 105 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human MDA-MB-453 cells, rat brain tissue, mouse brain tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human LOXL2. It differs from the mouse and rat sequences at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places lysyl oxidase homolog 2 in the nucleus, chromosome and endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed in many tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haobo Wang et al. (2024) Pulling the Rug Out from Under: Biomechanical Microenvironment Remodeling for Induction of Hepatic Stellate Cell Quiescence. Advanced Materials. 10.1002/adma.202406590
Fan Zhiqin et al. (2023) Phosphorylation of AKT by lysyl oxidase-like 2 activates the PI3K/AKT signaling pathway to promote proliferation, invasion and metastasis in esophageal squamous carcinoma. Clinical & Translational Oncology. 10.1007/s12094-023-03133-5
Yingmin Liu et al. (2023) LOXL2 promotes tumor proliferation and metastasis by FAK/Src signaling in esophageal squamous cell carcinoma. Electronic Journal of Biotechnology. 10.1016/j.ejbt.2023.01.002
