| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acyl-CoA:lysophosphatidylglycerol acyltransferase 1; 2-acylglycerophosphocholine O-acyltransferase; Acyl-CoA:monoacylglycerol acyltransferase LPGAT1; Lysophospholipid acyltransferase 7; LPLAT7; Stearoyl-CoA:1-lyso-2-acyl-PE acyltransferase; LPGAT1; FAM34A; KIAA0205 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LPGAT1 recombinant protein (Position: A32-Q348). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes acyl-CoA:lysophosphatidylglycerol acyltransferase 1 (gene LPGAT1) in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 43.1 kDa, and the supplier reports an observed band at about 43 kDa.
LPGAT1 is an endoplasmic reticulum acyltransferase that remodels the fatty acid chains of membrane phospholipids. It preferentially adds saturated long-chain fatty acids, especially stearate, to the sn-1 position of lysophosphatidylethanolamine, shaping the composition of phosphatidylethanolamine and phosphatidylcholine. LPGAT1 is studied in phospholipid metabolism and liver lipid homeostasis.
| Target | Acyl-CoA:lysophosphatidylglycerol acyltransferase 1 (gene LPGAT1; UniProt Q92604, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 43.1 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human LPGAT1 fragment (Ala32–Gln348), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places acyl-CoA:lysophosphatidylglycerol acyltransferase 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Highly expressed in liver and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
