| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lipoma-preferred partner; LIM domain-containing preferred translocation partner in lipoma; LPP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human LPP, which shares 100% and 94.7% amino acid (aa) sequence identity with mouse and rat LPP, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes lipoma-preferred partner (gene LPP) in human, monkey, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 65.7 kDa, and the supplier reports an observed band at about 80 kDa.
LPP (lipoma-preferred partner) is a LIM-domain protein of the zyxin family that localizes to focal adhesions and cell–cell contacts. It can shuttle to the nucleus and is thought to relay signals from adhesion sites to gene transcription. LPP was first identified as a fusion partner of HMGA2 in lipomas and is studied in cell adhesion and migration.
| Target | Lipoma-preferred partner (gene LPP; UniProt Q93052, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 65.7 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human A549 cells, human HepG2 cells, human HeLa cells, human MDA-MB-453 cells, monkey COS-7 cells, rat ovary tissue, rat PC-12 cells, mouse ovary tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue, human rectal cancer tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Immunogen
Synthetic peptide from the C-terminal region of human LPP. Sequence identity with the mouse and rat orthologs: 100% and 94.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places lipoma-preferred partner in the nucleus, cytoplasm, cell junction and cell membrane. Tissue expression noted by UniProt (human): Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
