| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeat and calponin homology domain-containing protein 4; Leucine-rich repeat neuronal protein 4; Leucine-rich neuronal protein; LRCH4; LRN; LRRN1; LRRN4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LRCH4 recombinant protein (Position: K256-R580). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes leucine-rich repeat and calponin homology domain-containing protein 4 (gene LRCH4) in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 73.5 kDa, and the supplier reports an observed band at about 84 kDa.
LRCH4 is a leucine-rich repeat and calponin homology domain protein that tunes signaling through several Toll-like receptors. In macrophages it helps lipopolysaccharide settle correctly into lipid raft membranes and may help keep these rafts intact, which makes it of interest in studies of innate immune activation and inflammatory responses.
| Target | Leucine-rich repeat and calponin homology domain-containing protein 4 (gene LRCH4; UniProt O75427, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 73.5 kDa |
| Observed MW | 84 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human SH-SY5Y cells, human MCF-7 cells, human K562 cells, human Caco-2 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human LRCH4 fragment (Lys256–Arg580), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places leucine-rich repeat and calponin homology domain-containing protein 4 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
