| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeats and immunoglobulin-like domains protein 2; LIG-2; LRIG2; KIAA0806; LIG2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LRIG2 recombinant protein (Position: E108-R977). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets leucine-rich repeats and immunoglobulin-like domains protein 2 (gene LRIG2) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 119.0 kDa, and the supplier reports an observed band at about 119 kDa.
LRIG2 is a member of the LRIG family of transmembrane proteins, which carry leucine-rich repeats and immunoglobulin-like domains. Its function is less well defined than that of its relative LRIG1, and it is studied mainly for its possible role in regulating receptor tyrosine kinase signaling.
| Target | Leucine-rich repeats and immunoglobulin-like domains protein 2 (gene LRIG2; UniProt O94898, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 119.0 kDa |
| Observed MW | 119 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human LRIG2 fragment (Glu108–Arg977), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places leucine-rich repeats and immunoglobulin-like domains protein 2 in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Detected in all tissues analyzed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
