| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3; LRIT3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LRIT3 recombinant protein (Position: E50-R573). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3 (gene LRIT3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 74.8 kDa, and the supplier reports an observed band at about 75 kDa.
LRIT3 is a leucine-rich repeat, immunoglobulin-like and transmembrane protein of the retina. It is needed for signal transfer from photoreceptors to ON-bipolar cells and for placing the TRPM1 cation channel at bipolar cell dendrite tips, so loss of LRIT3 disrupts ON-pathway transmission; it has also been linked to FGFR trafficking, and it is studied in retinal synapse biology and congenital stationary night blindness.
| Target | Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3 (gene LRIT3; UniProt Q3SXY7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 74.8 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human Jurkat cells, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Recombinant human LRIT3 fragment (Glu50–Arg573), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3 in the cell projection, perikaryon and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Detected in the outer plexiform layer (OPL) of the retina where it localizes to ON-bipolar cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
