| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prolow-density lipoprotein receptor-related protein 1;LRP-1;Alpha-2-macroglobulin receptor;A2MR;Apolipoprotein E receptor;APOER;CD91;Low-density lipoprotein receptor-related protein 1 85 kDa subunit;LRP-85;Low-density lipoprotein receptor-related protein 1 515 kDa subunit;LRP-515;Low-density lipoprotein receptor-related protein 1 intracellular domain;LRPICD;LRP1;A2MR, APR; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LRP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BDC-12 is a rabbit monoclonal antibody that recognizes prolow-density lipoprotein receptor-related protein 1 (gene LRP1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 504.6 kDa, and the supplier reports an observed band at about 85 kDa.
LRP1 is a large endocytic receptor of the LDL receptor family that binds dozens of ligands, including apolipoprotein E-containing lipoproteins, alpha-2-macroglobulin complexes and protease-inhibitor complexes. It clears these ligands from plasma and tissues, removes apoptotic cells, and also takes up tau protein, so it is studied in lipid metabolism, vascular biology and neurodegeneration research.
| Target | Prolow-density lipoprotein receptor-related protein 1 (gene LRP1; UniProt Q07954, human) |
|---|---|
| Clone | BDC-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 504.6 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human U87 cells, human HepG2 cells, human U2OS cells, human HeLa cells, rat brain tissue, rat heart muscle tissue, mouse brain tissue, human liver carcinoma tissue, human pituitary tumor tissue, mouse heart tissue, human prostate cancer tissue, human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human LRP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places prolow-density lipoprotein receptor-related protein 1 in the membrane and nucleus. Tissue expression noted by UniProt (human): Most abundant in liver, brain and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yaqi Qiu et al. (2023) CD44s-activated tPA/LRP1-NFκB pathway drives lamellipodia outgrowth in luminal-type breast cancer cells. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2023.1224827
Xiaorui Lv et al. (2023) Human Neural Stem Cell Secretome Inhibits Neuron Heme Uptake and Ferroptosis in Intracerebral Hemorrhage Through Nrf-2 Signaling Pathway. Stem Cells and Development. 10.1089/scd.2023.0010
Xuexun Zheng et al. (2026) Interaction between LRP1 and PAI-1 in lower extremity deep venous thrombosis. Thrombosis Research. 10.1016/j.thromres.2026.109645
Wang Ning-Ning et al. (2013) Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line. Asian Pacific Journal of Cancer Prevention. 10.7314/APJCP.2013.14.9.4983
