| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low-density lipoprotein receptor-related protein 5;LRP-5;LRP5;LR3, LRP7; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets low-density lipoprotein receptor-related protein 5 (gene LRP5) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 179.1 kDa, and the supplier reports an observed band at about 179 kDa.
LRP5 is a single-pass transmembrane coreceptor that partners with Frizzled receptors to transmit canonical Wnt signals. Ligand binding recruits Axin to LRP5, stabilizing β-catenin and switching on TCF/LEF target genes; through this route LRP5 controls bone mass by regulating osteoblasts and, with norrin and FZD4, supports retinal blood vessel development.
| Target | Low-density lipoprotein receptor-related protein 5 (gene LRP5; UniProt O75197, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 179.1 kDa |
| Observed MW | 179 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue, NIH cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human LRP5. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places low-density lipoprotein receptor-related protein 5 in the membrane and endoplasmic reticulum. Tissue expression noted by UniProt (human): Widely expressed, with the highest level of expression in the liver and in aorta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhong Gang et al. (2018) Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway. Cellular Physiology and Biochemistry. 10.1159/000495926
