| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich PPR motif-containing protein, mitochondrial; 130 kDa leucine-rich protein; LRP 130; GP130; LRPPRC; LRP130 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LRPPRC/GP130 recombinant protein (Position: A1281-S1394). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects leucine-rich PPR motif-containing protein, mitochondrial (gene LRPPRC) in human, mouse, rat and monkey samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 157.9 kDa, and the supplier reports an observed band at about 158 kDa.
LRPPRC is a leucine-rich pentatricopeptide repeat protein found mainly in mitochondria, with a smaller nuclear pool. In mitochondria it binds polyadenylated mRNAs and supports the stability and translation of mitochondrially encoded cytochrome c oxidase subunits, while in the nucleus it has been linked to mRNA export; it is studied in oxidative phosphorylation and mitochondrial disease, including a French-Canadian form of Leigh syndrome.
| Target | Leucine-rich PPR motif-containing protein, mitochondrial (gene LRPPRC; UniProt P42704, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 157.9 kDa |
| Observed MW | 158 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells, human HeLa cells, human Jurkat cells, human Caco-2 cells, monkey kidney tissue, monkey heart tissue, rat heart tissue, rat skeletal muscle tissue, rat kidney tissue, rat liver tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectal cancer tissue, mouse intestine tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): A431 cells, C6 cells.
Immunogen
Recombinant human LRPPRC/GP130 fragment (Ala1281–Ser1394), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and monkey. UniProt places leucine-rich PPR motif-containing protein, mitochondrial in the mitochondrion, nucleus, nucleus inner membrane and nucleus outer membrane. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Han Lei et al. (2024) Integrated analysis and experiments uncover the function of disulfidptosis in predicting immunotherapy effectiveness and delineating immune landscapes in uterine corpus endometrial carcinoma. Frontiers in Immunology. 10.3389/fimmu.2024.1454730
Shen Liming et al. (2021) Proteomic Profiling of Cerebrum Mitochondria, Myelin Sheath, and Synaptosome Revealed Mitochondrial Damage and Synaptic Impairments in Association with 3 × Tg‐AD Mice Model. Cellular and Molecular Neurobiology. 10.1007/s10571-021-01052-z
