| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1;Dardarin;LRRK2;PARK8; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LRRK2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AGB-12 is a rabbit monoclonal antibody that recognizes leucine-rich repeat serine/threonine-protein kinase 2 (gene LRRK2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 286.1 kDa, and the supplier reports an observed band at about 280 kDa.
LRRK2 is a large multidomain protein that combines a GTPase domain with a serine/threonine kinase domain. It phosphorylates a subset of Rab GTPases such as RAB8A and RAB10, shaping vesicle trafficking, lysosome function, autophagy and ciliogenesis; activating mutations in LRRK2 are a common genetic cause of Parkinson's disease, making it a major target in neurodegeneration research.
| Target | Leucine-rich repeat serine/threonine-protein kinase 2 (gene LRRK2; UniProt Q5S007, human) |
|---|---|
| Clone | AGB-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 286.1 kDa |
| Observed MW | 280 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): U87-MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human thyroid tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human LRRK2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leucine-rich repeat serine/threonine-protein kinase 2 in the cytoplasmic vesicle, perikaryon, Golgi apparatus membrane and cell projection. Tissue expression noted by UniProt (human): Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
