| Field | Specification |
|---|---|
| Target | |
| Alternative names | U6 snRNA-associated Sm-like protein LSm2; Protein G7b; Small nuclear ribonuclear protein D homolog; snRNP core Sm-like protein Sm-x5; LSM2; C6orf28; G7B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human LSM2, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects U6 snRNA-associated Sm-like protein LSm2 in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 10.8 kDa, and the supplier reports an observed band at about 11 kDa.
LSM2 is an Sm-like protein that forms part of the heptameric LSM2–8 ring, which binds the uridine-rich 3′ end of U6 snRNA. Through this ring it takes part in the U4/U6.U5 tri-snRNP and early spliceosome assembly, so it is studied in pre-mRNA splicing.
| Target | U6 snRNA-associated Sm-like protein LSm2 (gene LSM2; UniProt Q9Y333, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 10.8 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human SW620 cells, human Hek293 cells, human K562 cells, human Caco-2 cells, monkey Cos-7 cells, human Raji cells, human A431 cells, human U2OS cells, rat brain tissue, rat testis tissue, rat small intestines tissue, rat C6 cells, mouse testis tissue, mouse Neuro-2a cells, mouse Hepal-6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human adrenal carcinoma tissue, human lung cancer tissue, human rectal cancer tissue, human appendicitis tissue, human thyroid cancer tissue, human liver cancer tissue, rat lung tissue, mouse lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Synthetic peptide from the C-terminal region of human LSM2. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places U6 snRNA-associated Sm-like protein LSm2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
