| Field | Specification |
|---|---|
| Target | |
| Alternative names | U6 snRNA-associated Sm-like protein LSm8; LSM8 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LSM8 recombinant protein (Position: M1-H96). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6B11 is a mouse monoclonal antibody that recognizes U6 snRNA-associated Sm-like protein LSm8 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 10.4 kDa, and the supplier reports an observed band at about 16 kDa.
LSM8 is the Sm-like subunit that distinguishes the nuclear LSM2–8 ring from the cytoplasmic LSM1–7 ring. The LSM2–8 complex binds the uridine-rich 3′ end of U6 snRNA and supports assembly of the U4/U6.U5 tri-snRNP and spliceosome, so LSM8 is studied in pre-mRNA splicing.
| Target | U6 snRNA-associated Sm-like protein LSm8 (gene LSM8; UniProt O95777, human) |
|---|---|
| Clone | 6B11 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 10.4 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HL-60 cells, human Jurkat cells, human THP-1 whole, human K562 cells, rat testis tissue, rat pancrease tissue, mouse testis tissue, mouse pancrease tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human renal clear cells carcinoma tissue, human colonic adenocarcinoma tissue, human placenta tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): Jurkat cells, RH35 cells, RAW264.7 cells.
Immunogen
Recombinant human LSM8 fragment (Met1–His96), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places U6 snRNA-associated Sm-like protein LSm8 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
