| Field | Specification |
|---|---|
| Target | |
| Alternative names | Leukotriene A-4 hydrolase; LTA-4 hydrolase; Leukotriene A(4) hydrolase; Tripeptide aminopeptidase LTA4H; LTA4H; LTA4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanLTA4Hrecombinantprotein(Position:Q137-D611).HumanLTA4Hshares92.8%and93.7%aminoacid(aa)sequenceidentitywithmouseandratLTA4H,respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets leukotriene A-4 hydrolase (gene LTA4H) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 69.3 kDa, and the supplier reports an observed band at about 69 kDa.
Leukotriene A4 hydrolase (LTA4H) is a zinc-dependent enzyme with two activities. Its epoxide hydrolase activity converts leukotriene A4 into the neutrophil chemoattractant leukotriene B4, while its aminopeptidase activity breaks down the collagen-derived chemotactic peptide Pro-Gly-Pro, so it can both drive and limit inflammation and is studied in lipid mediator and inflammation research.
| Target | Leukotriene A-4 hydrolase (gene LTA4H; UniProt P09960, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 69.3 kDa |
| Observed MW | 69 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human Jurkat cells, human A549 cells, human HeLa cells, human HepG2 cells, human PC-3 cells, rat thymus tissue, rat C6 cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous carcinoma tissue, human liver cancer tissue, human rectal cancer tissue, human thyroid papillary carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Samples with a confirmed signal (flow cytometry): RT4 cells.
Immunogen
E. coli-derivedhumanLTA4Hrecombinantprotein(Position:Q137-D611).HumanLTA4Hshares92.8%and93.7%aminoacid(aa)sequenceidentitywithmouseandratLTA4H,respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places leukotriene A-4 hydrolase in the cytoplasm. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
