| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived mouse LY6C recombinant protein (Position: L27-G109). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-LY6C Antibody Picoband® is an antibody for Ly6c1/2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Ly6c1/2 (NADH dehydrogenase 2, mitochondrial); UniProt: P0CW02; NCBI Gene: 17067/100041546
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 14 kDa, calculated 14377 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-LY6C Antibody Picoband® catalog # A33313.
Biological background
Biological context: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Expression and localization notes: cellular localization: Mitochondrion inner membrane. Multi-pass membrane protein., tissue context: Ubiquitously expressed..
Common research applications
- Western blotting (WB): Compare Ly6c1/2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Ly6c1/2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Ly6c1/2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion inner membrane. Multi-pass membrane protein.
- Tissue details: Ubiquitously expressed.
- Research category: Chemokines,Immunology,Innate Immunity
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
