| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lymphocyte antigen 6D; Ly-6D; E48 antigen; LY6D; E48 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LY6D recombinant protein (Position: L21-L111). Human LY6D shares 60.4% amino acid (aa) sequence identity with mouse LY6D. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes lymphocyte antigen 6D (gene LY6D) in human samples and is validated for Western blot, IHC and ELISA.
Lymphocyte antigen 6D (LY6D) is a GPI-anchored Ly6-family surface protein. In early lymphoid development its expression marks the first stage of commitment of progenitors toward the B-cell lineage, and it is also found on some epithelial cells.
| Target | Lymphocyte antigen 6D (gene LY6D; UniProt Q14210, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human LY6D fragment (Leu21–Leu111), expressed in E. coli. Sequence identity with the mouse ortholog: 60.4%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places lymphocyte antigen 6D in the cell membrane. Tissue expression noted by UniProt (human): Expressed exclusively at the outer cell surface of transitional epithelia and the keratinocyte of stratified squamous epithelia.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
