Anti-LY6E/SCA-2 Antibody Picoband®

Overview

Anti-LY6E/SCA-2 Antibody Picoband® is an antibody for LY6E detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: LY6E (CD72 molecule); UniProt: Q16553
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 14 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-LY6E/SCA-2 Antibody Picoband® catalog # A09496-2.

Biological background

Biological context: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Expression and localization notes: cellular localization: Nucleus ., tissue context: Pre-B-cells and B-cells but not terminally differentiated plasma cells..

Common research applications

• Western blotting (WB): Compare LY6E levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of LY6E in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify LY6E-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Lymphocyte antigen 6E is a protein that in humans is encoded by the LY6E gene. Increased expression of Ly6E is associated with poor survival outcome in multiple malignancies as determined by a survey of more than 130 published clinical studies of gene expression studies on cancer tissue samples and adjacent normal tissues. Ly6E is associated with drug resistance and tumor immune escape in breast cancer. Further research is required to validate Ly6E for translation research.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus .
• Tissue details: Pre-B-cells and B-cells but not terminally differentiated plasma cells.
• Research category: Epigenetics and Nuclear Signaling,Mediator Complex,Transcription

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.