| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calponin-1;Basic calponin;Calponin H1, smooth muscle;CNN1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human LY75 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-LY75 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFAB-12; isotype Rabbit IgG; reactivity: Human,Mouse. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-LY75 Monoclonal Antibody catalog # M08183. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: LY75 (Calponin-1).
- Antibody format: Monoclonal; clone AFAB-12; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
LY75 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). . Reported cellular localization context: Secreted. Released from the secretory granules upon mast cell activation. . Tissue expression notes (as provided): Smooth muscle, and tissues containing significant amounts of smooth muscle.
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,B Cells,Cell Type Markers,Immunology,Non-CD,Tags & Cell Markers,Tumor Associated.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate LY75 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect LY75 expression by Western blot in cell or tissue lysates, Detect LY75 in FFPE tissue sections by immunohistochemistry, Localize LY75 by immunofluorescence/immunocytochemistry in cultured cells, Enrich LY75 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 34 kDa; calculated MW: 33170 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 34 kDa
- Cellular localization (provided): Secreted. Released from the secretory granules upon mast cell activation. .
- Tissue details (provided): Smooth muscle, and tissues containing significant amounts of smooth muscle.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
