| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ly6/PLAUR domain-containing protein 6; LYPD6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanLYPD6recombinantprotein(Position:A23-C155).HumanLYPD6shares97%and96.2%aminoacid(aa)sequenceidentitywithmouseandratLYPD6,respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ly6/PLAUR domain-containing protein 6 (gene LYPD6) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 19.1 kDa, and the supplier reports an observed band at about 19 kDa.
LYPD6 is a Ly6/uPAR-family protein expressed in the brain that modulates nicotinic acetylcholine receptors. It reduces nicotine-induced Ca2+ entry and allosterically inhibits certain receptor subtypes, including α7, and it has also been reported to enhance Wnt/β-catenin signaling.
| Target | Ly6/PLAUR domain-containing protein 6 (gene LYPD6; UniProt Q86Y78, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 19.1 kDa |
| Observed MW | 19 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human RT4 cells, human MCF-7 cells, human SiHa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SH-SY5Y cells.
Immunogen
E. coli-derivedhumanLYPD6recombinantprotein(Position:A23-C155).HumanLYPD6shares97%and96.2%aminoacid(aa)sequenceidentitywithmouseandratLYPD6,respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes ly6/PLAUR domain-containing protein 6 as secreted and also located in the cytoplasm, cell membrane and synapse. Tissue expression noted by UniProt (human): Detected in the temporal cortex (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
