| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acyl-protein thioesterase 2; APT-2; Lysophospholipase II; LPL-II; LysoPLA II; Palmitoyl-protein hydrolase; LYPLA2; APT2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derivedhumanLYPLA2recombinantprotein(Position:H38-V231).HumanLYPLA2shares99%aminoacid(aa)sequenceidentitywithbothmouseandratLYPLA2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects acyl-protein thioesterase 2 (gene LYPLA2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 24.7 kDa, and the supplier reports an observed band at about 25 kDa.
Acyl-protein thioesterase 2 (LYPLA2, APT2) is a cytosolic depalmitoylating enzyme closely related to APT1. It removes fatty acids from S-acylated proteins such as GAP43, HRAS, Gα subunits and gasdermin D, and also hydrolyzes lysophospholipids and prostaglandin glycerol esters, so it is studied in dynamic protein palmitoylation and lipid signaling.
| Target | Acyl-protein thioesterase 2 (gene LYPLA2; UniProt O95372, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 24.7 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, human HepG2 cells, human HEL cells, rat brain tissue, rat stomach tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectum adenocarcinoma tissue, human esophageal squamous carcinoma tissue, mouse stomach tissue, rat stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): PC-3 cells.
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
E. coli-derivedhumanLYPLA2recombinantprotein(Position:H38-V231).HumanLYPLA2shares99%aminoacid(aa)sequenceidentitywithbothmouseandratLYPLA2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places acyl-protein thioesterase 2 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in various breast cancer cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
