| Field | Specification |
|---|---|
| Target | |
| Alternative names | LYR motif-containing protein 4; LYRM4; C6orf149; ISD11 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human LYRM4 recombinant protein (Position: M1-T91). Human LYRM4 shares 89% amino acid (aa) sequence identity with mouse LYRM4. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes LYR motif-containing protein 4 (gene LYRM4) in human, mouse and rat samples and is validated for Western blot and ELISA.
LYRM4 (also called ISD11) is a small mitochondrial protein of the LYR motif family that stabilizes the cysteine desulfurase NFS1. Together with the acyl carrier protein NDUFAB1 it forms part of the core complex that builds [2Fe-2S] clusters on the scaffold ISCU, the first step in mitochondrial iron–sulfur protein biogenesis.
| Target | LYR motif-containing protein 4 (gene LYRM4; UniProt Q9HD34, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human LYRM4 fragment (Met1–Thr91), expressed in E. coli. Sequence identity with the mouse ortholog: 89%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places LYR motif-containing protein 4 in the mitochondrion and nucleus. Tissue expression noted by UniProt (human): Reduced mRNA levels in Friedreich ataxia patients.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
