| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acyl-protein thioesterase 1; APT-1; hAPT1; Lysophospholipase 1; Lysophospholipase I; LPL-I; LysoPLA I; Palmitoyl-protein hydrolase; LYPLA1; APT1; LPL1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Lysophospholipase 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21L68 is a rabbit monoclonal antibody that recognizes acyl-protein thioesterase 1 (gene LYPLA1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 24.7 kDa, and the supplier reports an observed band at about 25 kDa.
Acyl-protein thioesterase 1 (LYPLA1, APT1) is a cytosolic enzyme that removes palmitate and other fatty acids from S-acylated cysteines of proteins such as Ras and Gα subunits. This depalmitoylation lets proteins cycle on and off membranes, so LYPLA1 is studied in protein lipidation, Ras signaling and membrane trafficking; it also has lysophospholipase activity.
| Target | Acyl-protein thioesterase 1 (gene LYPLA1; UniProt O75608, human) |
|---|---|
| Clone | 21L68 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.7 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human fetal liver.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide based on human Lysophospholipase 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places acyl-protein thioesterase 1 in the cytoplasm, cell membrane, nuclear membrane and endoplasmic reticulum. Tissue expression noted by UniProt (human): Platelets. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
