| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysozyme C;3.2.1.17;1,4-beta-N-acetylmuramidase C;LYZ;LZM; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Lysozyme. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects lysozyme C (gene LYZ) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 16.5 kDa, and the supplier reports an observed band at about 15–17 kDa.
Lysozyme C (LYZ) is a small antibacterial enzyme that cleaves the peptidoglycan of bacterial cell walls, especially in Gram-positive bacteria. It is abundant in tears, saliva, milk and mucus and is produced in large amounts by monocytes, macrophages and neutrophils, so it is a classic marker of innate defense and myeloid cells.
| Target | Lysozyme C (gene LYZ; UniProt P61626, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 16.5 kDa |
| Observed MW | 15–17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human, Mouse) |
Samples with a confirmed band (WB): human THP-1 cells, human HL-60 cells, rat lung tissuerat PC-12 whole cellmouse lung tissuemouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human ileum tissue, human colon organoid tissue, mouse ileum tissue, mouse ileum organoid tissue, mouse colon issue.
Immunogen
Synthetic peptide from the C-terminal region of human lysozyme.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes lysozyme C as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yulai Fang et al. (2025) Integration of Multi-Omics and Network Pharmacology Analysis Reveals the Mechanism of Qingchang Huashi Jianpi Bushen Formula in Repairing the Epithelial Barrier of Ulcerative Colitis. Journal of Inflammation Research. 10.2147/JIR.S510966
Cui-Ping Xu et al. (2007) Dynamic changes and mechanism of intestinal endotoxemia in partially hepatectomized rats. World Journal of Gastroenterology. 10.3748/wjg.v13.i26.3592
