| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lymphatic vessel endothelial hyaluronic acid receptor 1;LYVE-1;Cell surface retention sequence-binding protein 1;CRSBP-1;Extracellular link domain-containing protein 1;Hyaluronic acid receptor;LYVE1;CRSBP1, HAR, XLKD1;UNQ230/PRO263; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human LYVE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
Clone AOCO-12 is a rabbit monoclonal antibody that recognizes lymphatic vessel endothelial hyaluronic acid receptor 1 (gene LYVE1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 35.2 kDa, and the supplier reports an observed band at about 29 kDa.
LYVE1 is a hyaluronan receptor related to CD44 that is expressed mainly on lymphatic endothelial cells and on some tissue macrophages. It binds hyaluronan and is thought to help transport it and to support leukocyte entry into lymphatics, and it is one of the most widely used markers for identifying lymphatic vessels.
| Target | Lymphatic vessel endothelial hyaluronic acid receptor 1 (gene LYVE1; UniProt Q9Y5Y7, human) |
|---|---|
| Clone | AOCO-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.2 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human A549 cells, human K562 cells, human HepG2 cells, human HEL cells, rat C6 cells, rat PC-12 cells, mouse Neuro-2a cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human LYVE1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places lymphatic vessel endothelial hyaluronic acid receptor 1 in the cell membrane. Tissue expression noted by UniProt (human): Mainly expressed in endothelial cells lining lymphatic vessels. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuan Yujia et al. (2024) Gut microbiota-derived acetate promotes long-term recovery via angiogenesis guided by lymphatic ingrowth in aged stroke subjects. Frontiers in Neuroscience. 10.3389/fnins.2024.1398913
Du Li-Cheng et al. (2014) VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma. Reproductive Biology and Endocrinology. 10.1186/1477-7827-12-14
DONG HE HAN et al. (2010) Upregulation of hyaluronan and its binding receptors in an experimental model of chronic cyclosporine nephropathy. Nephrology. 10.1111/j.1440-1797.2009.01167.x
Ling Shiqi et al. (2008) Clinical and Experimental Research of Corneal Lymphangiogenesis after Keratoplasty. Ophthalmologica. 10.1159/000144030
