| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 4;Immune activation protein 2;ACT-2;ACT2;Macrophage inflammatory protein 1-beta;MIP-1-beta;Protein H400;SIS-gamma;Small-inducible cytokine A4;Ccl4;Mip1b, Scya4; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse CCL4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets C-C motif chemokine 4 (gene Ccl4) in mouse samples and is validated for Western blot. The predicted molecular weight is 10.2 kDa, and the supplier reports an observed band at about 15 kDa.
CCL4, also known as MIP-1β, is a CC chemokine produced by activated monocytes, macrophages and CD8+ T cells. It acts mainly through CCR5 to attract immune cells to sites of inflammation, and by occupying CCR5 it can block entry of CCR5-tropic HIV-1, which makes it a frequent target in immunology and HIV research.
| Target | C-C motif chemokine 4 (gene Ccl4; UniProt P14097, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 10.2 kDa |
| Observed MW | 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of mouse CCL4.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes C-C motif chemokine 4 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
