| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181; MAD1L1; MAD1; TXBP181 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAD1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22M92 is a rabbit monoclonal antibody that recognizes mitotic spindle assembly checkpoint protein MAD1 (gene MAD1L1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 83.1 kDa, and the supplier reports an observed band at about 80 kDa.
MAD1 (MAD1L1) is a core component of the spindle assembly checkpoint that localizes to unattached kinetochores. Bound to closed MAD2, it acts as a template that converts free MAD2 into its active form, helping generate the mitotic checkpoint complex that delays anaphase until every chromosome is properly attached, so it is studied in chromosome segregation and aneuploidy.
| Target | Mitotic spindle assembly checkpoint protein MAD1 (gene MAD1L1; UniProt Q9Y6D9, human) |
|---|---|
| Clone | 22M92 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.1 kDa |
| Observed MW | 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MAD1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitotic spindle assembly checkpoint protein MAD1 in the nucleus, chromosome, nucleus envelope and cytoplasm. Tissue expression noted by UniProt (human): Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level)... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
