| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic spindle assembly checkpoint protein MAD2B; Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7; MAD2L2; MAD2B; REV7 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Mad2L2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADFO-13 is a rabbit monoclonal antibody that recognizes mitotic spindle assembly checkpoint protein MAD2B (gene MAD2L2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 24.3 kDa, and the supplier reports an observed band at about 24 kDa.
MAD2L2, also known as REV7 or MAD2B, is an adaptor protein related to MAD2. It is an essential subunit of DNA polymerase zeta in translesion DNA synthesis and also belongs to the shieldin complex that limits DNA end resection during double-strand break repair, so it is studied in DNA damage tolerance, repair pathway choice and PARP-inhibitor responses.
| Target | Mitotic spindle assembly checkpoint protein MAD2B (gene MAD2L2; UniProt Q9UI95, human) |
|---|---|
| Clone | ADFO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.3 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:40 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human mad2L2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitotic spindle assembly checkpoint protein MAD2B in the nucleus, cytoplasm and chromosome. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Kejun et al. (2023) Identification of a Novel CD8+ T cell exhaustion-related gene signature for predicting survival in hepatocellular carcinoma. BMC Cancer. 10.1186/s12885-023-11648-x
